Hotspot

rps3

LSC · 85598-86257 · 660 bp

Pi 0.00909

MarkerSeek score 49.10

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 660
variable_sites: 6
pi: 0.00909
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TTCCATCGTTCCCATTGCTT	TCTGGGTTTCAACGAAGCAA	45.0	45.0	57.8	58.0	1595 bp	1.0	78.9
rps3_p2	TTCCATCGTTCCCATTGCTT	TCCCGGGCATCTACCATTAT	45.0	50.0	57.8	58.0	2023 bp	1.0	78.8
rps3_p3	CCATCGTTCCCATTGCTTCT	TCTGGGTTTCAACGAAGCAA	50.0	45.0	58.2	58.0	1593 bp	1.0	78.6
rps3_p4	CCATCGTTCCCATTGCTTCT	TCCCGGGCATCTACCATTAT	50.0	50.0	58.2	58.0	2021 bp	1.0	78.5
rps3_p5	TCCATCGTTCCCATTGCTTC	TCCCGGGCATCTACCATTAT	50.0	50.0	58.2	58.0	2022 bp	1.0	78.5