Hotspot

rbcL

LSC · 57822-59249 · 1428 bp

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Pi 0.0063
MarkerSeek score 62.40
Primer available yes
Label rbcL

Feature Metadata

label_name
rbcL
feature_type
gene
region
LSC
length_bp
1428
variable_sites
9
pi
0.0063
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL_p1 TGAGTTGTAGGGAGGGACTT AGAATCATTGGGTTCGGCTC 50.0 50.0 57.3 58.0 1539 bp 1.0 74.4
rbcL_p2 TGAGTTGTAGGGAGGGACTT AATCATTGGGTTCGGCTCAA 50.0 45.0 57.3 57.8 1537 bp 1.0 73.0
rbcL_p3 AGACCTTGTTCTTGCGAGAA AGAATCATTGGGTTCGGCTC 45.0 50.0 57.1 58.0 1570 bp 1.0 72.8
rbcL_p4 TGAGTTGTAGGGAGGGACTT GAATCATTGGGTTCGGCTCA 50.0 50.0 57.3 58.2 1538 bp 1.0 72.7
rbcL_p5 TGAGTTGTAGGGAGGGACTT TCATTGGGTTCGGCTCAATC 50.0 50.0 57.3 58.2 1535 bp 1.0 72.7