Hotspot

psbC

LSC · 35630-37051 · 1422 bp

Pi 0.00492

MarkerSeek score 61.60

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 7
pi: 0.00492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTCGTTTCCCAGGAAATCCG	GACCCGCTTCCCTCAATATG	50.0	55.0	58.2	58.1	1611 bp	1.0	78.0
psbC_p2	TTCGTTTCCCAGGAAATCCG	TAAGTATGACCCGCTTCCCT	50.0	50.0	58.2	57.8	1618 bp	1.0	77.5
psbC_p3	TTCGTTTCCCAGGAAATCCG	AGCATAAGCATTGGAACCCC	50.0	50.0	58.2	58.2	1561 bp	1.0	77.1
psbC_p4	TTCGTTTCCCAGGAAATCCG	GCATAAGCATTGGAACCCCT	50.0	50.0	58.2	58.2	1560 bp	1.0	77.1
psbC_p5	GCAGCGGAAGATCCTGAATT	GACCCGCTTCCCTCAATATG	50.0	55.0	58.3	58.1	1590 bp	1.0	76.8