Hotspot

trnW-trnP

LSC · 69278-69460 · 183 bp

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Pi 0.0176
MarkerSeek score 63.90
Primer available yes
Label trnW-trnP

Feature Metadata

label_name
trnW-trnP
feature_type
igs
region
LSC
length_bp
183
variable_sites
3
pi
0.0176
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-trnP_p1 AAGACGACAAGAGCGGAATC CACGGGTTCAAATCCTGTCA 50.0 50.0 58.0 58.1 288-301 bp 1.0 83.5
trnW-trnP_p2 AAGACGACAAGAGCGGAATC GTCACGGGTTCAAATCCTGT 50.0 50.0 58.0 58.1 290-303 bp 1.0 83.4
trnW-trnP_p3 AAGACGACAAGAGCGGAATC TGTCACGGGTTCAAATCCTG 50.0 50.0 58.0 58.1 291-304 bp 1.0 83.4
trnW-trnP_p4 AAGACGACAAGAGCGGAATC GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 301-314 bp 1.0 83.3
trnW-trnP_p5 AAGACGACAAGAGCGGAATC TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 302-315 bp 1.0 83.3