Hotspot

psbE-petL

LSC · 67415-68685 · 1271 bp

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Pi 0.0142
MarkerSeek score 59.50
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1271
variable_sites
18
pi
0.0142
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GACCAATAAATAGAGCCGAGGT 50.0 45.5 57.9 57.7 1448 bp 1.0 61.9
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC GACCAATAAATAGAGCCGAGGT 50.0 45.5 57.8 57.7 1371 bp 1.0 61.2
psbE-petL_p3 ACGAATAACCAACCCGCAAT GACCAATAAATAGAGCCGAGGT 45.0 45.5 58.2 57.7 1457 bp 1.0 61.0
psbE-petL_p4 TGCTGACGAATAACCAACCC GACCAATAAATAGAGCCGAGGT 50.0 45.5 58.2 57.7 1462 bp 1.0 60.9
psbE-petL_p5 CGAATAACCAACCCGCAATG GACCAATAAATAGAGCCGAGGT 50.0 45.5 57.8 57.7 1456 bp 1.0 60.8