Hotspot

petA-psbJ

LSC · 65561-66629 · 1069 bp

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Pi 0.0112
MarkerSeek score 62.30
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1069
variable_sites
12
pi
0.0112
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GCATCTGTTGTTTTGGCACA CCGGATTAGGTTCGTCCTTG 45.0 55.0 57.8 58.1 1176 bp 1.0 78.6
petA-psbJ_p2 TTGGCATCTGTTGTTTTGGC CCGGATTAGGTTCGTCCTTG 45.0 55.0 57.8 58.1 1179 bp 1.0 78.5
petA-psbJ_p3 GCATCTGTTGTTTTGGCACA TCCGGATTAGGTTCGTCCTT 45.0 50.0 57.8 58.1 1177 bp 1.0 78.0
petA-psbJ_p4 TTGGCATCTGTTGTTTTGGC TCCGGATTAGGTTCGTCCTT 45.0 50.0 57.8 58.1 1180 bp 1.0 77.8
petA-psbJ_p5 TGTCCAAGGCCTTTTGTTCT CCGGATTAGGTTCGTCCTTG 45.0 55.0 57.5 58.1 1201 bp 1.0 76.7