Hotspot

ycf4-cemA

LSC · 63101-63669 · 569 bp

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Pi 0.0516
MarkerSeek score 80.50
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
569
variable_sites
29
pi
0.0516
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TGACTCCGCGAGAAATTGAG AGATCCACCAGGGCAAAAAG 50.0 50.0 58.0 58.1 722-1086 bp 1.0 82.6
ycf4-cemA_p2 TGACTCCGCGAGAAATTGAG GAGATCCACCAGGGCAAAAA 50.0 50.0 58.0 58.1 723-1087 bp 1.0 82.5
ycf4-cemA_p3 TGACTCCGCGAGAAATTGAG GATCCACCAGGGCAAAAAGA 50.0 50.0 58.0 58.1 721-1085 bp 1.0 82.5
ycf4-cemA_p4 TTGGCCTATTTCTTGCGTGT AGATCCACCAGGGCAAAAAG 45.0 50.0 58.1 58.1 687-1051 bp 1.0 82.0
ycf4-cemA_p5 TTGGCCTATTTCTTGCGTGT GATCCACCAGGGCAAAAAGA 45.0 50.0 58.1 58.1 686-1050 bp 1.0 81.9