Hotspot

trnT-psbD

LSC · 33378-34620 · 1243 bp

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Pi 0.0185
MarkerSeek score 62.00
Primer available yes
Label trnT-psbD

Feature Metadata

label_name
trnT-psbD
feature_type
igs
region
LSC
length_bp
1243
variable_sites
23
pi
0.0185
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-psbD_p1 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1393-1395 bp 1.0 78.8
trnT-psbD_p2 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1396-1398 bp 1.0 78.7
trnT-psbD_p3 ACGGCCCTTTTAACTCAGTG ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1410-1412 bp 1.0 78.5
trnT-psbD_p4 GTGGTAGAGTAACGCCATGG CCCTTCGTAACCAGTCATCC 55.0 55.0 58.1 57.7 1377-1379 bp 1.0 78.3
trnT-psbD_p5 TCAGTGGTAGAGTAACGCCA CCCTTCGTAACCAGTCATCC 50.0 55.0 58.1 57.7 1380-1382 bp 1.0 78.2