Hotspot

rpoB-trnC

LSC · 28172-29138 · 967 bp

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Pi 0.0103
MarkerSeek score 64.70
Primer available yes
Label rpoB-trnC

Feature Metadata

label_name
rpoB-trnC
feature_type
igs
region
LSC
length_bp
967
variable_sites
10
pi
0.0103
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC_p1 ATTCCCGCATTTCCATCTCC GGCGAGTTATACCAGCCAAA 50.0 50.0 58.0 58.0 1113-1186 bp 1.0 80.3
rpoB-trnC_p2 ATTCCCGCATTTCCATCTCC AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.0 58.4 1037-1110 bp 1.0 77.8
rpoB-trnC_p3 GCATTTCCATCTCCGAGCAT GGCGAGTTATACCAGCCAAA 50.0 50.0 58.4 58.0 1107-1180 bp 1.0 77.3
rpoB-trnC_p4 ACATTCCCGCATTTCCATCT GGCGAGTTATACCAGCCAAA 45.0 50.0 57.6 58.0 1115-1188 bp 1.0 76.9
rpoB-trnC_p5 AGACATTCCCGCATTTCCAT GGCGAGTTATACCAGCCAAA 45.0 50.0 57.6 58.0 1117-1190 bp 1.0 76.9