Hotspot

trnS-trnG

LSC · 9108-9867 · 760 bp

Back to Lovoa

Pi 0.0146
MarkerSeek score 55.80
Primer available yes
Label trnS-trnG

Feature Metadata

label_name
trnS-trnG
feature_type
igs
region
LSC
length_bp
760
variable_sites
11
pi
0.0146
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-trnG_p1 TAAGCCACTCAGCCATCTCT CGAACGAATCACACTTTTACCA 50.0 40.9 58.1 57.3 819-838 bp 1.0 58.7
trnS-trnG_p2 TAAGCCACTCAGCCATCTCT TCGAACGAATCACACTTTTACC 50.0 40.9 58.1 57.0 820-839 bp 1.0 56.6
trnS-trnG_p3 GCTTTAAGCCACTCAGCCAT CGAACGAATCACACTTTTACCA 50.0 40.9 58.5 57.3 823-842 bp 1.0 55.4
trnS-trnG_p4 ACTCGTACACCGGATTAGCA CGAACGAATCACACTTTTACCA 50.0 40.9 58.5 57.3 850-869 bp 1.0 55.4
trnS-trnG_p5 CTCGTACACCGGATTAGCAA CGAACGAATCACACTTTTACCA 50.0 40.9 57.4 57.3 849-868 bp 1.0 55.1