Hotspot

trnR-UCU-trnG-UCC

LSC · 51045-51249 · 205 bp

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Pi 0.0821
MarkerSeek score 71.70
Primer available yes
Label trnR-UCU-trnG-UCC

Feature Metadata

label_name
trnR-UCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
205
variable_sites
35
pi
0.0821
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.90
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnG-UCC_p1 TGCGCCCAATAGGATTTGAA AGCCTTCCAAGCTAACGATG 45.0 50.0 57.9 58.0 239-329 bp 1.0 90.7
trnR-UCU-trnG-UCC_p2 TTGCGCCCAATAGGATTTGA AGCCTTCCAAGCTAACGATG 45.0 50.0 57.9 58.0 240-330 bp 1.0 90.7
trnR-UCU-trnG-UCC_p3 TGCGCCCAATAGGATTTGAA ACCCTTAGCCTTCCAAGCTA 45.0 50.0 57.9 58.0 245-335 bp 1.0 90.6
trnR-UCU-trnG-UCC_p4 TTGCGCCCAATAGGATTTGA ACCCTTAGCCTTCCAAGCTA 45.0 50.0 57.9 58.0 246-336 bp 1.0 90.6
trnR-UCU-trnG-UCC_p5 GCGCCCAATAGGATTTGAAC AGCCTTCCAAGCTAACGATG 50.0 50.0 57.5 58.0 238-328 bp 1.0 87.8