Hotspot

psbA-trnK-UUU

LSC · 1454-1745 · 292 bp

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Pi 0.0404
MarkerSeek score 74.50
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
292
variable_sites
29
pi
0.0404
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.96
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 CCCCATAGGTTTTCGCTCTC CGACTAGTTCCGGGTTCAAG 55.0 55.0 58.0 58.0 319-394 bp 1.0 89.8
psbA-trnK-UUU_p2 CCCCATAGGTTTTCGCTCTC CTAGTTCCGGGTTCAAGTCC 55.0 55.0 58.0 57.6 316-391 bp 1.0 86.8
psbA-trnK-UUU_p3 CCCATAGGTTTTCGCTCTCG CGACTAGTTCCGGGTTCAAG 55.0 55.0 58.4 58.0 318-393 bp 1.0 86.7
psbA-trnK-UUU_p4 GCTCTCGCGTCTCTCTAAAA CGACTAGTTCCGGGTTCAAG 50.0 55.0 57.5 58.0 305-380 bp 1.0 86.0
psbA-trnK-UUU_p5 TACAGAAGCGACCCCATAGG CGACTAGTTCCGGGTTCAAG 55.0 55.0 58.6 58.0 330-405 bp 1.0 85.2