Hotspot

atpI

LSC · 16069-16812 · 744 bp

Back to Loropetalum

Pi 0.00134

MarkerSeek score 59.50

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 1
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	TGCGTTGGGCTAAGCTAAAA	TCTATTTCGGTCAGAGGGCA	45.0	50.0	58.1	58.1	807 bp	1.0	78.2
atpI_p2	TTGCGTTGGGCTAAGCTAAA	TCTATTTCGGTCAGAGGGCA	45.0	50.0	58.1	58.1	808 bp	1.0	78.2
atpI_p3	ATTGCGTTGGGCTAAGCTAA	TCTATTTCGGTCAGAGGGCA	45.0	50.0	57.9	58.1	809 bp	1.0	78.0
atpI_p4	TCTTGAGCCACGCATACATT	TCTATTTCGGTCAGAGGGCA	45.0	50.0	57.6	58.1	826 bp	1.0	75.8
atpI_p5	CTTGAGCCACGCATACATTG	TCTATTTCGGTCAGAGGGCA	50.0	50.0	57.5	58.1	825 bp	1.0	75.3