Hotspot

atpA

LSC · 11346-12869 · 1524 bp

Back to Loropetalum

Pi 0.000656

MarkerSeek score 59.10

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 1
pi: 0.000656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	TGATTTGAATCAGACACACTTCT	TGTTTGGGGCGATGAAAGAA	34.8	45.0	56.1	58.0	1726 bp	1.0	41.0
atpA_p2	TGATTTGAATCAGACACACTTCT	ATGTTTGGGGCGATGAAAGA	34.8	45.0	56.1	57.8	1727 bp	1.0	40.0
atpA_p3	TGATTTGAATCAGACACACTTCT	GGTATGTTTGGGGCGATGAA	34.8	50.0	56.1	58.2	1730 bp	1.0	40.0
atpA_p4	TGATTTGAATCAGACACACTTC	TGTTTGGGGCGATGAAAGAA	36.4	45.0	54.7	58.0	1726 bp	1.0	40.0
atpA_p5	TGATTTGAATCAGACACACTTCT	ATTGGTATGTTTGGGGCGAT	34.8	45.0	56.1	57.5	1733 bp	1.0	40.0