Hotspot

accD

LSC · 60914-62416 · 1503 bp

Back to Loropetalum

Pi 0.000665

MarkerSeek score 59.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1503
variable_sites: 1
pi: 0.000665
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCCGAGTGATTGGATCTATATGC	CGGATTTTGACTTTGATTCCGA	43.5	40.9	57.7	57.3	1654 bp	1.0	40.0
accD_p2	TCCGAGTGATTGGATCTATATGC	TCGGATTTTGACTTTGATTCCG	43.5	40.9	57.7	57.3	1655 bp	1.0	40.0
accD_p3	CCGAGTGATTGGATCTATATGCT	CGGATTTTGACTTTGATTCCGA	43.5	40.9	57.7	57.3	1653 bp	1.0	40.0
accD_p4	CCGAGTGATTGGATCTATATGCT	TCGGATTTTGACTTTGATTCCG	43.5	40.9	57.7	57.3	1654 bp	1.0	40.0
accD_p5	TCCGAGTGATTGGATCTATATGC	TGTCACCAAAGAAAACTCCA	43.5	40.0	57.7	55.0	1679 bp	1.0	40.0