Hotspot

rbcL-accD

LSC · 60291-60913 · 623 bp

Back to Loropetalum

Pi 0

MarkerSeek score 58.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 623
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CCGTGAAGCTAGCAAATGGA	ACATCGAATTGAACCACCGT	50.0	45.0	58.3	57.8	795-796 bp	1.0	76.6
rbcL-accD_p2	TCCGTGAAGCTAGCAAATGG	ACATCGAATTGAACCACCGT	50.0	45.0	58.3	57.8	796-797 bp	1.0	76.6
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	ACATCGAATTGAACCACCGT	55.0	45.0	57.7	57.8	777-778 bp	1.0	76.3
rbcL-accD_p4	CCGTGAAGCTAGCAAATGGA	TAGAGCTTTCTTGCCCCCTA	50.0	50.0	58.3	57.7	768-769 bp	1.0	75.9
rbcL-accD_p5	TCCGTGAAGCTAGCAAATGG	TAGAGCTTTCTTGCCCCCTA	50.0	50.0	58.3	57.7	769-770 bp	1.0	75.9