Hotspot

ndhC-trnV-UAC

LSC · 53925-55138 · 1214 bp

Back to Loropetalum

Pi 0.000824
MarkerSeek score 59.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1214
variable_sites
1
pi
0.000824
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGCTAATCGGAGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1354 bp 1.0 75.9
ndhC-trnV-UAC_p2 GTCCTTTGCTAATCGGAGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 1353 bp 1.0 73.2
ndhC-trnV-UAC_p3 CTGGTCCTTTGCTAATCGGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 1357 bp 1.0 72.9
ndhC-trnV-UAC_p4 TCTGGTCCTTTGCTAATCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 1358 bp 1.0 72.9
ndhC-trnV-UAC_p5 TGGTCCTTTGCTAATCGGAG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 1356 bp 1.0 72.9