Hotspot

trnF-GAA-ndhJ

LSC · 51529-52241 · 713 bp

Back to Loropetalum

Pi 0

MarkerSeek score 60.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 713
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	846-855 bp	1.0	80.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	810-819 bp	1.0	79.8
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	ACGCCTGAAACGCATCTTAA	55.0	45.0	58.0	58.2	874-883 bp	1.0	79.2
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	834-843 bp	1.0	79.1
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ACGCCTGAAACGCATCTTAA	50.0	45.0	58.1	58.2	838-847 bp	1.0	78.9