Hotspot

atpH-atpI

LSC · 14926-16068 · 1143 bp

Back to Loropetalum

Pi 0.000875

MarkerSeek score 59.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1143
variable_sites: 1
pi: 0.000875
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.1	57.8	1198 bp	1.0	77.8
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	1256 bp	1.0	77.8
atpH-atpI_p3	CAGCAGCAATAACAGAAGCG	CGAATCCATGGAGGGTCATC	50.0	55.0	57.8	57.8	1208 bp	1.0	77.3
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	CGAATCCATGGAGGGTCATC	55.0	55.0	58.2	57.8	1260 bp	1.0	77.1
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	CGAATCCATGGAGGGTCATC	50.0	55.0	57.8	57.8	1215 bp	1.0	76.9