Hotspot

rps16-trnQ-UUG

LSC · 6331-7991 · 1661 bp

Back to Loropetalum

Pi 0
MarkerSeek score 58.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1661
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1740-1741 bp 1.0 79.4
rps16-trnQ-UUG_p2 GTCTTTCAAGTCGCACGTTG TACAAATCGAAATGGGGCGT 50.0 45.0 58.0 57.9 1800-1801 bp 1.0 79.1
rps16-trnQ-UUG_p3 ATGTCTTTCAAGTCGCACGT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1742-1743 bp 1.0 78.4
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1733-1734 bp 1.0 78.3
rps16-trnQ-UUG_p5 ATGTCTTTCAAGTCGCACGT TACAAATCGAAATGGGGCGT 45.0 45.0 58.1 57.9 1802-1803 bp 1.0 78.0