Hotspot

trnH-GUG-psbA

LSC · 94-472 · 379 bp

Back to Loropetalum

Pi 0.00264
MarkerSeek score 55.70
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
379
variable_sites
1
pi
0.00264
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG ATGCATGAACGTAACGCTCA 55.0 45.0 58.1 58.3 473 bp 1.0 76.9
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TGCATGAACGTAACGCTCAT 55.0 45.0 58.1 58.3 472 bp 1.0 76.9
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA ATGCATGAACGTAACGCTCA 50.0 45.0 57.9 58.3 487 bp 1.0 76.9
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA TGCATGAACGTAACGCTCAT 50.0 45.0 57.9 58.3 486 bp 1.0 76.9
trnH-GUG-psbA_p5 AATCCACTGCCTTGATCCAC TGCATGAACGTAACGCTCAT 50.0 45.0 57.9 58.3 485 bp 1.0 76.9