Hotspot

ycf1

SSC · 96406-101424 · 5019 bp

Back to Loranthus

Pi 0.019

MarkerSeek score 56.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5019
variable_sites: 207
pi: 0.019
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGAGCAAGCGGAATGGTATC	CCGATTAGCCGAGGATCATG	50.0	55.0	58.0	58.0	432 bp	1.0	84.6
ycf1_p2	TGAGCAAGCGGAATGGTATC	CGATTAGCCGAGGATCATGG	50.0	55.0	58.0	58.0	431 bp	1.0	84.6
ycf1_p3	CAAGCGGAATGGTATCCCAA	CCGATTAGCCGAGGATCATG	50.0	55.0	57.9	58.0	428 bp	0.666667	68.3
ycf1_p4	CAAGCGGAATGGTATCCCAA	CGATTAGCCGAGGATCATGG	50.0	55.0	57.9	58.0	427 bp	0.666667	68.3
ycf1_p5	GCGGAATGGTATCCCAAGTT	CGATTAGCCGAGGATCATGG	50.0	55.0	57.9	58.0	424 bp	0.666667	68.3