Hotspot

trnL-CAA-ndhB

IRb · 80207-80962 · 756 bp

Back to Loranthus

Pi 0.0067

MarkerSeek score 64.50

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 756
variable_sites: 12
pi: 0.0067
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	TCCGTCGACAAAAGGATCAC	50.0	50.0	58.0	57.9	801-836 bp	1.0	84.5
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	TCCGTCGACAAAAGGATCAC	50.0	50.0	58.0	57.9	799-834 bp	1.0	84.5
trnL-CAA-ndhB_p3	TACCATTTCACCACCAAGGC	TCCGTCGACAAAAGGATCAC	50.0	50.0	58.1	57.9	789-824 bp	1.0	83.9
trnL-CAA-ndhB_p4	GCGTGTCTACCATTTCACCA	TCCGTCGACAAAAGGATCAC	50.0	50.0	58.2	57.9	796-831 bp	1.0	83.0
trnL-CAA-ndhB_p5	TCTCGCGTGTCTACCATTTC	TCCGTCGACAAAAGGATCAC	50.0	50.0	57.7	57.9	800-835 bp	1.0	82.4