Hotspot

psbT-psbN

LSC · 60963-61029 · 67 bp

Back to Loranthus

Pi 0.0557

MarkerSeek score 74.90

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 67
variable_sites: 8
pi: 0.0557
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	AACCGCCTAAAGTTCCAACA	GCCCTCTCAACAACTACGAG	45.0	55.0	57.6	58.0	148-154 bp	1.0	82.0
psbT-psbN_p2	ACCGCCTAAAGTTCCAACAA	GCCCTCTCAACAACTACGAG	45.0	55.0	57.6	58.0	147-153 bp	1.0	82.0
psbT-psbN_p3	AACCGCCTAAAGTTCCAACA	AGATCCATTCGACGAACACG	45.0	50.0	57.6	58.1	130-136 bp	1.0	81.9
psbT-psbN_p4	ACCGCCTAAAGTTCCAACAA	AGATCCATTCGACGAACACG	45.0	50.0	57.6	58.1	129-135 bp	1.0	81.9
psbT-psbN_p5	TCGAGAACCGCCTAAAGTTC	GCCCTCTCAACAACTACGAG	50.0	55.0	57.6	58.0	153-159 bp	1.0	81.7