Hotspot

ycf4-cemA

LSC · 49238-49372 · 135 bp

Back to Loranthus

Pi 0.0486

MarkerSeek score 75.90

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 135
variable_sites: 15
pi: 0.0486
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	AAGTCGCGGAATTGGCTTAT	GAGAAAGATCCACAAGGGCA	45.0	50.0	58.0	57.5	248-260 bp	1.0	84.4
ycf4-cemA_p2	AGTCGCGGAATTGGCTTATT	GAGAAAGATCCACAAGGGCA	45.0	50.0	58.0	57.5	247-259 bp	1.0	84.4
ycf4-cemA_p3	AAAGTCGCGGAATTGGCTTA	GAGAAAGATCCACAAGGGCA	45.0	50.0	58.2	57.5	249-261 bp	1.0	83.2
ycf4-cemA_p4	AAGTCGCGGAATTGGCTTAT	AGAGAAAGATCCACAAGGGC	45.0	50.0	58.0	57.2	249-261 bp	1.0	82.0
ycf4-cemA_p5	AGTCGCGGAATTGGCTTATT	AGAGAAAGATCCACAAGGGC	45.0	50.0	58.0	57.2	248-260 bp	1.0	82.0