Hotspot

atpB-rbcL

LSC · 43855-44671 · 817 bp

Back to Loranthus

Pi 0.0126

MarkerSeek score 63.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 22
pi: 0.0126
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GCTACATCCAATACCGGACC	AACACCGGCTTTGAATCCAA	55.0	45.0	57.8	58.3	961-979 bp	1.0	82.1
atpB-rbcL_p2	GCTACATCCAATACCGGACC	TAACACCGGCTTTGAATCCA	55.0	45.0	57.8	57.4	962-980 bp	1.0	79.9
atpB-rbcL_p3	GGGGGAAAAGCTACATCCAA	AACACCGGCTTTGAATCCAA	50.0	45.0	57.5	58.3	970-988 bp	1.0	79.2
atpB-rbcL_p4	TTTTCAGGCACAGAAACCCC	AACACCGGCTTTGAATCCAA	50.0	45.0	58.6	58.3	910-928 bp	1.0	78.8
atpB-rbcL_p5	GCTACATCCAATACCGGACC	CCGGCTTTGAATCCAACACT	55.0	50.0	57.8	58.8	957-975 bp	1.0	78.5