Hotspot

ycf3-trnS-GGA

LSC · 37953-38536 · 584 bp

Back to Loranthus

Pi 0.0304
MarkerSeek score 71.70
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
584
variable_sites
38
pi
0.0304
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TGCCTCCCTTTCTCTTGAAG CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 733-763 bp 1.0 80.4
ycf3-trnS-GGA_p2 GCCTCCCTTTCTCTTGAAGT CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 732-762 bp 1.0 80.4
ycf3-trnS-GGA_p3 TGCCTCCCTTTCTCTTGAAG TTCCAATGCTACGCCTTGAA 50.0 45.0 57.1 57.8 704-734 bp 1.0 79.7
ycf3-trnS-GGA_p4 GCCTCCCTTTCTCTTGAAGT TTCCAATGCTACGCCTTGAA 50.0 45.0 57.1 57.8 703-733 bp 1.0 79.7
ycf3-trnS-GGA_p5 GCCTCCCTTTCTCTTGAAGTT CCCTCGGTAAACAAAAGCCT 47.6 50.0 57.9 58.1 732-762 bp 1.0 78.1