Hotspot

psbC-trnS-UGA

LSC · 29235-29401 · 167 bp

Back to Loranthus

Pi 0.0311

MarkerSeek score 68.50

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 167
variable_sites: 11
pi: 0.0311
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCGTAGGTCATTTGTGGCAC	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.2	58.1	354-355 bp	1.0	80.4
psbC-trnS-UGA_p2	TCGTAGGTCATTTGTGGCAC	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.2	58.2	300-301 bp	1.0	79.6
psbC-trnS-UGA_p3	GCAGCAGGATTTGAAAAGGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.6	58.1	313-314 bp	1.0	79.3
psbC-trnS-UGA_p4	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	317-318 bp	1.0	79.3
psbC-trnS-UGA_p5	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	316-317 bp	1.0	78.8