Hotspot

petN-psbM

LSC · 23267-24118 · 852 bp

Back to Loranthus

Pi 0.018

MarkerSeek score 69.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 852
variable_sites: 31
pi: 0.018
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GTATGGGGGAGAAGTGGACT	ACGTAAAAACGGCTAGTCAA	55.0	40.0	58.1	55.4	595-915 bp	1.0	64.8
petN-psbM_p2	AGTATGGGGGAGAAGTGGAC	ACGTAAAAACGGCTAGTCAA	55.0	40.0	58.1	55.4	596-916 bp	1.0	64.7
petN-psbM_p3	AGTATGGGGGAGAAGTGGAC	TCCTTTACGTAAAAACGGCT	55.0	40.0	58.1	55.3	602-922 bp	1.0	64.5
petN-psbM_p4	GTATGGGGGAGAAGTGGACT	TCCTTTACGTAAAAACGGCT	55.0	40.0	58.1	55.3	601-921 bp	1.0	64.5
petN-psbM_p5	TATGGGGGAGAAGTGGACTC	ACGTAAAAACGGCTAGTCAA	55.0	40.0	57.8	55.4	594-914 bp	1.0	64.3