Hotspot

ycf46

Genome · 4788-6251 · 1464 bp

Back to Lophurella

Pi 0.0719

MarkerSeek score 80.00

Primer available yes

Label ycf46

Feature Metadata

label_name: ycf46
feature_type: gene
region: Genome
length_bp: 1464
variable_sites: 201
pi: 0.0719
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf46_p1	AAACCATTCCGAACTTGGCT	AATTAGACGGGGCCAAAGAC	45.0	50.0	58.0	57.9	2230 bp	0.2	47.0
ycf46_p2	ATCTCCCCCAAGAGTTCACA	AATTAGACGGGGCCAAAGAC	50.0	50.0	58.0	57.9	2728 bp	0.2	46.6
ycf46_p3	TCTCCCCCAAGAGTTCACAT	AATTAGACGGGGCCAAAGAC	50.0	50.0	58.0	57.9	2727 bp	0.2	46.6
ycf46_p4	CAAGTCGAGCAGAGACGAAA	AATTAGACGGGGCCAAAGAC	50.0	50.0	57.9	57.9	2828 bp	0.2	46.6
ycf46_p5	CAAACCATTCCGAACTTGGC	AATTAGACGGGGCCAAAGAC	50.0	50.0	57.9	57.9	2231 bp	0.2	46.5