Hotspot

ycf37

Genome · 6541-7032 · 492 bp

Back to Lophurella

Pi 0.112

MarkerSeek score 76.50

Primer available yes

Label ycf37

Feature Metadata

label_name: ycf37
feature_type: gene
region: Genome
length_bp: 492
variable_sites: 117
pi: 0.112
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf37_p1	ACCACTTTTTGTTTTCATCCGA	TGGTTGGGTAAGGAGACCAT	36.4	50.0	57.0	57.9	1233-1245 bp	0.4	37.7
ycf37_p2	GACCACTTTTTGTTTTCATCCGA	TGGTTGGGTAAGGAGACCAT	39.1	50.0	58.1	57.9	1234-1246 bp	0.4	37.1
ycf37_p3	ACCACTTTTTGTTTTCATCCGA	TTGGTTGGGTAAGGAGACCA	36.4	50.0	57.0	58.2	1234-1246 bp	0.4	36.7
ycf37_p4	ACCACTTTTTGTTTTCATCCGA	GACTTTGTGACTCGGTTGGT	36.4	50.0	57.0	58.1	1249 bp	0.2	23.7
ycf37_p5	GACCACTTTTTGTTTTCATCCGA	GACTTTGTGACTCGGTTGGT	39.1	50.0	58.1	58.1	1250 bp	0.2	23.0