Hotspot

ycf29

Genome · 154019-154639 · 621 bp

Back to Lophurella

Pi 0.0651
MarkerSeek score 80.20
Primer available yes
Label ycf29

Feature Metadata

label_name
ycf29
feature_type
gene
region
Genome
length_bp
621
variable_sites
90
pi
0.0651
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf29_p1 TGTTGATTCTTTACTTGGCCA AGGCAATGGATTGTGACTCC 38.1 50.0 55.7 57.9 1201 bp 0.6 43.4
ycf29_p2 TGTTGATTCTTTACTTGGCCA GGCAATGGATTGTGACTCCA 38.1 50.0 55.7 58.2 1200 bp 0.6 43.1
ycf29_p3 TGTTGATTCTTTACTTGGCCAA AGGCAATGGATTGTGACTCC 36.4 50.0 56.4 57.9 1201 bp 0.6 41.4
ycf29_p4 TTGTTGATTCTTTACTTGGCCA AGGCAATGGATTGTGACTCC 36.4 50.0 56.4 57.9 1202 bp 0.6 41.4
ycf29_p5 TGTTGATTCTTTACTTGGCCAA GGCAATGGATTGTGACTCCA 36.4 50.0 56.4 58.2 1200 bp 0.6 41.2