Hotspot

ycf23

Genome · 46023-46871 · 849 bp

Back to Lophurella

Pi 0.0869

MarkerSeek score 81.40

Primer available yes

Label ycf23

Feature Metadata

label_name: ycf23
feature_type: gene
region: Genome
length_bp: 849
variable_sites: 155
pi: 0.0869
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf23_p1	AATGTGTTCGTGCTTGTCCT	ACATAAAAGCAGGCGACATCA	45.0	42.9	58.0	58.3	1537 bp	0.2	37.4
ycf23_p2	ATGTGTTCGTGCTTGTCCTT	ACATAAAAGCAGGCGACATCA	45.0	42.9	58.0	58.3	1536 bp	0.2	37.4
ycf23_p3	CAATGTGTTCGTGCTTGTCC	ACATAAAAGCAGGCGACATCA	50.0	42.9	58.0	58.3	1538 bp	0.2	37.4
ycf23_p4	TCAAATAGCCTCCGCTCCTA	ACATAAAAGCAGGCGACATCA	50.0	42.9	57.9	58.3	1473 bp	0.2	37.0
ycf23_p5	GACGGTTGTAAAGCTGGTCA	ACATAAAAGCAGGCGACATCA	50.0	42.9	58.1	58.3	1490 bp	0.2	36.7