Hotspot

syfB

Genome · 156265-158283 · 2019 bp

Back to Lophurella

Pi 0.0913

MarkerSeek score 78.10

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: Genome
length_bp: 2019
variable_sites: 395
pi: 0.0913
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	TGCCACAGAAATATACCGCC	CGAATCCTCCTACCCCAGAT	50.0	55.0	58.0	58.0	2773-2781 bp	1.0	87.8
syfB_p2	TCGGTAAGAGCGTACCAGAT	CGAATCCTCCTACCCCAGAT	50.0	55.0	58.0	58.0	2725-2733 bp	1.0	87.7
syfB_p3	GTTCGGTAAGAGCGTACCAG	CGAATCCTCCTACCCCAGAT	55.0	55.0	58.1	58.0	2727-2735 bp	1.0	87.4
syfB_p4	TGCCACAGAAATATACCGCC	GATCACGTGACGGTAGTACG	50.0	55.0	58.0	57.9	2641 bp	0.2	47.0
syfB_p5	TCGGTAAGAGCGTACCAGAT	GATCACGTGACGGTAGTACG	50.0	55.0	58.0	57.9	2593 bp	0.2	47.0