Hotspot

dsbD

Genome · 23192-23929 · 738 bp

Back to Lophurella

Pi 0.0898

MarkerSeek score 78.80

Primer available yes

Label dsbD

Feature Metadata

label_name: dsbD
feature_type: gene
region: Genome
length_bp: 738
variable_sites: 140
pi: 0.0898
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dsbD_p1	ATCCTTCCTGGGGAGTTTCA	AGGAGCAATACGACCATACA	50.0	45.0	57.9	56.0	1336 bp	0.2	31.9
dsbD_p2	TCAAATCCTTCCTGGGGAGT	AGGAGCAATACGACCATACA	50.0	45.0	57.9	56.0	1340 bp	0.2	31.9
dsbD_p3	GGTTCAAATCCTTCCTGGGG	AGGAGCAATACGACCATACA	55.0	45.0	58.1	56.0	1343 bp	0.2	31.2
dsbD_p4	TCCTTCCTGGGGAGTTTCAA	AGGAGCAATACGACCATACA	50.0	45.0	58.2	56.0	1335 bp	0.2	31.0
dsbD_p5	TACCAATTTCACCACGTCCG	AGGAGCAATACGACCATACA	50.0	45.0	58.2	56.0	1541 bp	0.2	30.7