Hotspot

carA

Genome · 13041-14210 · 1170 bp

Back to Lophurella

Pi 0.0643

MarkerSeek score 81.00

Primer available yes

Label carA

Feature Metadata

label_name: carA
feature_type: gene
region: Genome
length_bp: 1170
variable_sites: 160
pi: 0.0643
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
carA_p1	AATGGTTATGGTCCGATGCC	GTGGACGATAGATGCACCAG	50.0	55.0	58.0	58.1	1468-1489 bp	0.6	70.9
carA_p2	AATGGTTATGGTCCGATGCC	GCACCAGAAGGACACTTACC	50.0	55.0	58.0	58.2	1455-1476 bp	0.6	70.4
carA_p3	AATGGTTATGGTCCGATGCC	GATGCACCAGAAGGACACTT	50.0	50.0	58.0	57.8	1458-1479 bp	0.6	70.4
carA_p4	AATGGTTATGGTCCGATGCC	TGGACGATAGATGCACCAGA	50.0	50.0	58.0	58.2	1467-1488 bp	0.6	70.2
carA_p5	AATGGTTATGGTCCGATGCC	ATGTGGACGATAGATGCACC	50.0	50.0	58.0	57.5	1470-1491 bp	0.6	67.7