Hotspot

accD

Genome · 10928-11797 · 870 bp

Back to Lophurella

Pi 0.0524

MarkerSeek score 79.30

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 870
variable_sites: 97
pi: 0.0524
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TGGAGCTGGTTTAACATGGG	TCTCTAGCAGGAGTCGCTAA	50.0	50.0	57.8	57.3	1725-1736 bp	1.0	81.1
accD_p2	ACTGCTCCTCATCCTAAGCT	TCTCTAGCAGGAGTCGCTAA	50.0	50.0	57.8	57.3	2073 bp	0.2	40.9
accD_p3	ACTGCTCCTCATCCTAAGCT	CACCGTTATGACATTGAGCG	50.0	50.0	57.8	57.3	2003 bp	0.2	40.9
accD_p4	TGGAGCTGGTTTAACATGGG	CACCGTTATGACATTGAGCG	50.0	50.0	57.8	57.3	1656 bp	0.2	40.5
accD_p5	ACTGCTCCTCATCCTAAGCT	CGTTATGACATTGAGCGCAA	50.0	45.0	57.8	57.2	2000 bp	0.2	40.2