Hotspot

trnM-cemA

Genome · 66322-66508 · 187 bp

Back to Lophurella

Pi 0.198

MarkerSeek score 81.30

Primer available yes

Label trnM-cemA

Feature Metadata

label_name: trnM-cemA
feature_type: igs
region: Genome
length_bp: 187
variable_sites: 68
pi: 0.198
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-cemA_p1	TAAAAACGGGTCGCACATGA	TCTCCTTCTGCTGTTGCTAC	45.0	50.0	58.1	57.2	283-408 bp	1.0	85.0
trnM-cemA_p2	TAAAAACGGGTCGCACATGA	CTCCTTCTGCTGTTGCTACT	45.0	50.0	58.1	57.2	282-407 bp	1.0	85.0
trnM-cemA_p3	CTAAAAACGGGTCGCACATG	TCTCCTTCTGCTGTTGCTAC	50.0	50.0	57.8	57.2	284-409 bp	1.0	84.1
trnM-cemA_p4	CTAAAAACGGGTCGCACATG	CTCCTTCTGCTGTTGCTACT	50.0	50.0	57.8	57.2	283-408 bp	1.0	84.0
trnM-cemA_p5	TAAAAACGGGTCGCACATGA	CTCCTTCTGCTGTTGCTACTT	45.0	47.6	58.1	57.9	282-407 bp	1.0	82.6