Hotspot

psbA

Genome · 371-1432 · 1062 bp

Back to Lophophora

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: Genome
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACCGATTTCTTGGTCTGTCA	TGCTTGGGAGTCCCTGATAA	45.0	50.0	56.8	58.0	1196 bp	1.0	70.0
psbA_p2	ACCGATTTCTTGGTCTGTCA	TTGCTTGGGAGTCCCTGATA	45.0	50.0	56.8	58.0	1197 bp	1.0	70.0
psbA_p3	ACCGATTTCTTGGTCTGTCA	AATTCGTGTGCTTTGCTTGG	45.0	45.0	56.8	57.9	1209 bp	1.0	69.1
psbA_p4	ACCGATTTCTTGGTCTGTCAA	TGCTTGGGAGTCCCTGATAA	42.9	50.0	57.5	58.0	1196 bp	1.0	67.8
psbA_p5	ACCGATTTCTTGGTCTGTCAA	TTGCTTGGGAGTCCCTGATA	42.9	50.0	57.5	58.0	1197 bp	1.0	67.8