Hotspot

rpl32-trnL-UAG

Genome · 97542-98732 · 1191 bp

Back to Lophophora

Pi 0
MarkerSeek score 58.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
Genome
length_bp
1191
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCCGGGAATTCAACTTTGTT ATACGATGTCATGCCGCTAC 40.0 50.0 55.5 57.9 1374-1375 bp 1.0 59.2
rpl32-trnL-UAG_p2 TTCCGGGAATTCAACTTTGT ATACGATGTCATGCCGCTAC 40.0 50.0 55.5 57.9 1375-1376 bp 1.0 59.2
rpl32-trnL-UAG_p3 TCCGGGAATTCAACTTTGTT CTCGCACTGCTTCCTAAGAG 40.0 55.0 55.5 58.1 1333-1334 bp 1.0 59.1
rpl32-trnL-UAG_p4 TCCGGGAATTCAACTTTGTT CTCTCGCACTGCTTCCTAAG 40.0 55.0 55.5 58.1 1335-1336 bp 1.0 59.1
rpl32-trnL-UAG_p5 TTCCGGGAATTCAACTTTGT CTCGCACTGCTTCCTAAGAG 40.0 55.0 55.5 58.1 1334-1335 bp 1.0 59.1