Hotspot

clpP-trnS-GCU

Genome · 66214-66673 · 460 bp

Back to Lophophora

Pi 0.0366

MarkerSeek score 77.60

Primer available yes

Label clpP-trnS-GCU

Feature Metadata

label_name: clpP-trnS-GCU
feature_type: igs
region: Genome
length_bp: 460
variable_sites: 14
pi: 0.0366
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-trnS-GCU_p1	AACCCACGAGTCTTCTTCCT	CTTTCGTCCACTCAGCCATC	50.0	55.0	58.3	58.6	479-557 bp	1.0	78.8
clpP-trnS-GCU_p2	CCCACGAGTCTTCTTCCTCT	CTTTCGTCCACTCAGCCATC	55.0	55.0	58.4	58.6	477-555 bp	1.0	77.5
clpP-trnS-GCU_p3	AACCCACGAGTCTTCTTCCT	ACGGAAAAGGAAAGAGAGGG	50.0	50.0	58.3	57.1	549-627 bp	1.0	76.8
clpP-trnS-GCU_p4	AACCCACGAGTCTTCTTCCT	TTTCGTCCACTCAGCCATCT	50.0	50.0	58.3	59.0	478-556 bp	1.0	75.7
clpP-trnS-GCU_p5	CCCACGAGTCTTCTTCCTCT	ACGGAAAAGGAAAGAGAGGG	55.0	50.0	58.4	57.1	547-625 bp	1.0	75.4