Hotspot

rps18-atpB

Genome · 29377-30126 · 750 bp

Back to Lophophora

Pi 0

MarkerSeek score 59.00

Primer available yes

Label rps18-atpB

Feature Metadata

label_name: rps18-atpB
feature_type: igs
region: Genome
length_bp: 750
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-atpB_p1	TGAAAGAATCAAGCCGACTCT	CCTGGAGGAAAAGCTACGTC	42.9	55.0	57.3	58.0	976-978 bp	1.0	66.6
rps18-atpB_p2	TGAAAGAATCAAGCCGACTCT	AGCTACGTCTAGTACCGGAC	42.9	55.0	57.3	58.1	965-967 bp	1.0	66.3
rps18-atpB_p3	TGAAAGAATCAAGCCGACTCT	AAACCCCAGGACCTGAAGTA	42.9	50.0	57.3	57.9	900-902 bp	1.0	65.7
rps18-atpB_p4	TGAAAGAATCAAGCCGACTCT	ACGTCTAGTACCGGACCAAT	42.9	50.0	57.3	57.9	961-963 bp	1.0	65.6
rps18-atpB_p5	TGAAAGAATCAAGCCGACTCT	CTACGTCTAGTACCGGACCA	42.9	55.0	57.3	57.7	963-965 bp	1.0	64.2