Hotspot

ycf1

SSC · 128803-134343 · 5541 bp

Back to Lophira

Pi 0.000722

MarkerSeek score 52.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5541
variable_sites: 4
pi: 0.000722
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGAGCATTAATCCTTGGAA	TCTGACTGATAGAACAAACACA	40.0	36.4	54.3	55.1	280 bp	1.0	41.1
ycf1_p2	ATGGAGCATTAATCCTTGGA	TCTGACTGATAGAACAAACACA	40.0	36.4	54.0	55.1	281 bp	1.0	41.1
ycf1_p3	TGGAGCATTAATCCTTGGAA	TCTGACTGATAGAACAAACACAA	40.0	34.8	54.3	55.8	280 bp	1.0	41.1
ycf1_p4	ATGGAGCATTAATCCTTGGAA	TCTGACTGATAGAACAAACACA	38.1	36.4	54.9	55.1	281 bp	1.0	41.1
ycf1_p5	TGGAGCATTAATCCTTGGAAT	TCTGACTGATAGAACAAACACA	38.1	36.4	54.9	55.1	280 bp	1.0	41.1