Hotspot

rps8

LSC · 84202-84606 · 405 bp

Back to Lophira

Pi 0

MarkerSeek score 22.00

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 22.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	GTCCATTCCTGCGTAAACCA	CGATTGCCCGAGAATTGAGA	50.0	50.0	58.2	58.1	1452-1453 bp	1.0	78.3
rps8_p2	TTTTGACTCTATCCCCCGGT	CGATTGCCCGAGAATTGAGA	50.0	50.0	58.0	58.1	978 bp	0.5	59.2
rps8_p3	TTTTGACTCTATCCCCCGGT	TTGCCCGAGAATTGAGACAG	50.0	50.0	58.0	57.9	975 bp	0.5	58.9
rps8_p4	TTTTGACTCTATCCCCCGGT	GCCCGAGAATTGAGACAGTT	50.0	50.0	58.0	57.9	973 bp	0.5	58.9
rps8_p5	TTTTGACTCTATCCCCCGGT	AATTTTTGGTGCGATTGCCC	50.0	45.0	58.0	58.2	989 bp	0.5	58.1