Hotspot

psbA

LSC · 487-1548 · 1062 bp

Back to Lophira

Pi 0.00188

MarkerSeek score 47.80

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.00188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACTCATATACACTAAGACCAAAGTC	ACTTGGGAGTCCCTGATGAT	36.0	50.0	56.1	57.7	1128 bp	1.0	40.1
psbA_p2	ACTCATATACACTAAGACCAAAGTC	GTGTACTTGGGAGTCCCTGA	36.0	55.0	56.1	58.4	1132 bp	1.0	40.1
psbA_p3	ACTCATATACACTAAGACCAAAGT	ACTTGGGAGTCCCTGATGAT	33.3	50.0	55.0	57.7	1128 bp	1.0	40.1
psbA_p4	ACTCATATACACTAAGACCAAAGT	GTGTACTTGGGAGTCCCTGA	33.3	55.0	55.0	58.4	1132 bp	1.0	40.1
psbA_p5	ACTCATATACACTAAGACCAAAGTC	TGTACTTGGGAGTCCCTGAT	36.0	50.0	56.1	57.4	1131 bp	1.0	40.1