Hotspot

atpF

LSC · 13505-14774 · 1270 bp

Back to Lophira

Pi 0

MarkerSeek score 27.30

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1270
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GGGGGAAATAATGCCTACAGT	ACAACAAATCGATAATTACTCTGTCT	47.6	30.8	57.4	57.0	1344-1349 bp	1.0	40.0
atpF_p2	GGGGGAAATAATGCCTACAGT	ACAACAAATCGATAATTACTCTGTC	47.6	32.0	57.4	55.7	1344-1349 bp	1.0	40.0
atpF_p3	GGGGGAAATAATGCCTACAGT	ACAACAAATCGATAATTACTCTGT	47.6	29.2	57.4	54.6	1344-1349 bp	1.0	40.0
atpF_p4	GGGGGAAATAATGCCTACAGT	AACAACAAATCGATAATTACTCTGTCT	47.6	29.6	57.4	57.6	1345-1350 bp	1.0	40.0
atpF_p5	GGGGGAAATAATGCCTACAGT	AGAACAACAAATCGATAATTACTCTGT	47.6	29.6	57.4	57.6	1347-1352 bp	1.0	40.0