Hotspot

rpl2-rps19

IRa · 159539-159603 · 65 bp

Back to Lophira

Pi 0.0154

MarkerSeek score 61.30

Primer available yes

Label rpl2-rps19

Feature Metadata

label_name: rpl2-rps19
feature_type: igs
region: IRa
length_bp: 65
variable_sites: 1
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rps19_p1	GGGCCCCAATTGGTAGAAAA	CGGGACCGCGTTACTATTAT	50.0	50.0	58.1	57.3	291-292 bp	1.0	74.1
rpl2-rps19_p2	TTGATTCTTCGTCGCCGTAG	CGGGACCGCGTTACTATTAT	50.0	50.0	58.1	57.3	199-200 bp	1.0	73.9
rpl2-rps19_p3	TTGGGGTTATCCTGCACTTG	CGGGACCGCGTTACTATTAT	50.0	50.0	57.8	57.3	257-258 bp	1.0	72.9
rpl2-rps19_p4	ATTTGATTCTTCGTCGCCGT	CGGGACCGCGTTACTATTAT	45.0	50.0	58.3	57.3	201-202 bp	1.0	72.5
rpl2-rps19_p5	TTTGATTCTTCGTCGCCGTA	CGGGACCGCGTTACTATTAT	45.0	50.0	57.6	57.3	200-201 bp	1.0	71.9