Hotspot

rpl32-trnL

SSC · 117869-118923 · 1055 bp

Back to Lophira

Pi 0.00284

MarkerSeek score 60.40

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 1055
variable_sites: 3
pi: 0.00284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	GGGAAATCTCTTTCGACCGG	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.3	57.9	1205-1206 bp	1.0	76.4
rpl32-trnL_p2	GGAAATCTCTTTCGACCGGT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.6	57.9	1204-1205 bp	1.0	76.2
rpl32-trnL_p3	GGGAAATCTCTTTCGACCGG	AGCAGCGTGTCTACCAATTT	55.0	45.0	58.3	57.8	1198-1199 bp	1.0	75.9
rpl32-trnL_p4	GGGAAATCTCTTTCGACCGG	CCTAAGAGCAGCGTGTCTAC	55.0	55.0	58.3	57.8	1204-1205 bp	1.0	75.9
rpl32-trnL_p5	GGGAAATCTCTTTCGACCGG	CTAAGAGCAGCGTGTCTACC	55.0	55.0	58.3	57.8	1203-1204 bp	1.0	75.9