Hotspot

ndhF-rpl32

SSC · 116782-117661 · 880 bp

Back to Lophira

Pi 0.00114

MarkerSeek score 59.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 880
variable_sites: 1
pi: 0.00114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGCTGCAGGAAAAAGTAGGA	TTTCAACGCTGACCCATACC	45.0	50.0	57.1	58.2	1056-1060 bp	1.0	71.3
ndhF-rpl32_p2	AGGAAAAAGTAGGAGTCCCCA	TTTCAACGCTGACCCATACC	47.6	50.0	58.0	58.2	1050-1054 bp	1.0	70.9
ndhF-rpl32_p3	TGCTGCAGGAAAAAGTAGGA	CCGGTCGAAAGAGATTTCCC	45.0	55.0	57.1	58.3	1088-1092 bp	1.0	70.0
ndhF-rpl32_p4	TGCTGCAGGAAAAAGTAGGA	ACCGGTCGAAAGAGATTTCC	45.0	50.0	57.1	57.6	1089-1093 bp	1.0	69.7
ndhF-rpl32_p5	AGGAAAAAGTAGGAGTCCCCA	CCGGTCGAAAGAGATTTCCC	47.6	55.0	58.0	58.3	1082-1086 bp	1.0	69.6