Hotspot

psaJ-rpl33

LSC · 71064-71548 · 485 bp

Back to Lophira

Pi 0.00412

MarkerSeek score 54.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 485
variable_sites: 2
pi: 0.00412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTCCCAGATGCGTTGACA	TACTCGGGCATCTTTACCCT	45.0	50.0	58.0	57.8	553-554 bp	1.0	78.7
psaJ-rpl33_p2	TTTTCCCAGATGCGTTGACA	GCATCTTTACCCTTGGCCAT	45.0	50.0	58.0	58.2	546-547 bp	1.0	78.3
psaJ-rpl33_p3	ATGCGTTGACATTCCCCTTT	TACTCGGGCATCTTTACCCT	45.0	50.0	58.1	57.8	544-545 bp	1.0	78.3
psaJ-rpl33_p4	ATGCGTTGACATTCCCCTTT	GCATCTTTACCCTTGGCCAT	45.0	50.0	58.1	58.2	537-538 bp	1.0	77.9
psaJ-rpl33_p5	TGGTTCGGTTCTTTAGCAGG	TACTCGGGCATCTTTACCCT	50.0	50.0	57.8	57.8	596-597 bp	1.0	77.5